How to maintain the good quality of the sterilization?

  During the disinfection and sterilization, we should be noted the nature and characteristics of the method, and also pay attention to the usable method and external factors about the disinfection efficacy. 

    Regardless of which method we use to disinfection or sterilization, the disinfection effect will be affected by many factors, so if we can control and use the advantage of these factors, it can increase the disinfection effect. Otherwise, it will lead the disinfection or sterilization to failure.

The main factors are the following points:

1) Sterilize dose:

      The sterilize dose is the basic condition to kill microorganisms, include the strength and time. If increase the sterilize strength, also can increase the speed of the sterilization. While decrease the sterilization time, it also decrease the efficacy. Of course, if the sterilize strength is lower than the standard, it can’t reach the sterilize goal even if increase the time.

2)The types and quantities of microbial contamination:

     According to the different types of biology, so there are the different disinfection effects. In addition, the number of microbes also affect the result. So before the disinfection, we should be noted the types and quantities of microbes contamination. In general, the microbial resistance is stronger or more serious pollution, then the disinfection is also more difficult.

3) PH value:

      Changing the PH value can affect the results of some sterilizations. One side, the disinfectant will be impacted by the PH value, it result of increased or decreased the disinfectant activity. On the other hand, the PH value will impact the microorganism.

4) Antagonism:

    For the chemical disinfection, pay attention to the interference about the antagonistic substances. For example, the quaternaries effects will be neutralized by the soap or anionic detergents. And acidic or alkaline disinfectant will be neutralized by the alkaline or acidic substances, then decrease the disinfect efficacy.

5) Penetration:

   When the instruments are disinfected, the bactericidal factors must be applied to the microorganism itself directly. Otherwise, it won’t have the bactericidal effect. For example, UV disinfection effect only can apply the microorganisms on the surface and shallow liquid, a piece of paper can reduce 95% the bactericidal effect.

Finally, there are many factors will affect the efficacy of disinfection and sterilization. No matter which method we use, we must check whether there is any factor will affect the result or not.